Identification Of Lymphatic And Blood Vessels
The purpose of this paper was to investigate the reactivity of various antibodies with porcine intestinal tissue. Twelve different antibodies on three different sections of the small intestine were compared.
Abstract
The purpose of this paper was to investigate the reactivity of various antibodies with porcine intestinal tissue. Twelve different antibodies on three different sections of the small intestine were compared. Some antibodies were originally directed against human tissue, namely CIV22, C-3D5, HM19/2, KG 7/30, Lu-5 and PM-2K. The other clones PB1, PE1, PG1, PM1, PP1 and PP2 have been developed against porcine tissue. The antibodies developed against human tissue still work for swine tissue because the epitope (the motif recognized by the antibody) is the same in both species. Testing was done on the twelve following preparations:
- Duodenum, lengthwise and crosswise
- Jejunum, lengthwise and crosswise
- Ileum, lengthwise and crosswise
Every tissue probe was sectioned with a cryostat, brought onto a slide and fixed there. This was followed by indirect (two-step) staining with twelve different antibodies, each one in three different concentrations. Hereby the method of indirect staining was used. First a specific antibody is applied, after which a secondary antibody carrying the color-giving peroxidase was added. The secondary antibody docks onto the primary antibody, marking the cell or tissue structure. Through observation under a light microscope, the specific staining attributes of the antibodies and the quality of staining could be compared with each other. The results and discussion in this paper will focus on the jejunum cross sections, since it yielded the best results.
This work was performed entirely by Alexander Pfeifer, with material support from BMA Biomedicals, a division of Chemoforma AG. This publication is accessible through www.bma.ch and may be freely distributed citing ©Alexander Pfeifer.